Nonfaradaic Current Suppression in DNA-Based Electrochemical Assays with a Differential Potentiostat.

ABSTRACT: One of the key factors limiting sensitivity in many electrochemical assays is the nonfaradaic or capacitive current. This is particularly true in modern assay systems based on DNA monolayers at gold electrode surfaces, which have shown great promise for bioanalysis in complex milieu such as whole blood or serum. While various changes in analytical parameters, redox reporter molecules, DNA structures, probe coverage, and electrode surface area have been shown useful, background reduction by hardware subtraction has not yet been explored for these assays. Here, we introduce new electrochemistry hardware that considerably suppresses nonfaradaic currents through real-time analog subtraction during current-to-voltage conversion in the potentiostat. This differential potentiostat (DiffStat) conf

SUBMITTER: Holtan MD 

PROVIDER: S-EPMC6917968 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature