Evaluation of synthetic formaldehyde and methanol assimilation pathways in Yarrowia lipolytica.

ABSTRACT:

Background

Crude glycerol coming from biodiesel production is an attractive carbon source for biological production of chemicals. The major impurity in preparations of crude glycerol is methanol, which is toxic for most microbes. Development of microbes, which would not only tolerate the methanol, but also use it as co-substrate, would increase the feasibility of bioprocesses using crude glycerol as substrate.

Results

To prevent methanol conversion to CO₂ via formaldehyde and formate, the formaldehyde dehydrogenase (FLD) gene was identified in and deleted from Yarrowia lipolytica. The deletion strain was able to convert methanol to formaldehyde without expression of heterologous methanol dehydrogenases. Further, it was shown that expression of heterologous

SUBMITTER: Vartiainen E 

PROVIDER: S-EPMC6918578 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature