Single cell transcriptome dynamics from pluripotency to FLK1⁺ mesoderm.

ABSTRACT: Hemangiogenic progenitors generating blood and endothelial cells are specified from FLK1-expressing (FLK1⁺) mesoderm by the transcription factor ETV2. FLK1⁺ mesoderm also contributes to smooth muscle and cardiomyocytes. However, the developmental process of FLK1⁺ mesoderm generation and its allocation to various cell fates remain obscure. Recent single cell RNA-sequencing studies of early embryos or in vitro-differentiated human embryonic stem (ES) cells have provided unprecedented information on the spatiotemporal resolution of cells in embryogenesis. These snapshots, however, lack information on continuous dynamic developmental processes. Here, we performed single cell RNA sequencing of in vitro-differentiated mouse ES cells to capture the c

SUBMITTER: Zhao H 

PROVIDER: S-EPMC6918769 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature