Autophagy-related Djatg8 is required for remodeling in planarian Dugesia japonica.

ABSTRACT: Planarians are the earliest free-living platyhelminthe with triploblastic and bilateral-symmetry. As an integral component of tissue homeostasis and regeneration, remodeling occurs constantly in the general planarian life history. In the present study, we isolate three planarian Dugesia japonica Atg8 genes (Djatg8-1, Djatg8-2, Djatg8-3) that show high sequence similarity with Atg8 from yeast and human. Results from whole-mount in situ hybridization indicate that Djatg8-2 and Djatg8-3 are strongly expressed in blastemas during Dugesia japonica regeneration. Using RNA interference, inhibition of Djatg8-1 gene expression has no obvious effect on planarian morphological changes. Interestingly, downregulation of Djatg8-2</

SUBMITTER: Kang J 

PROVIDER: S-EPMC6918785 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature