Changes of tight junction and interleukin-8 expression using a human gastroid monolayer model of Helicobacter pylori infection.

ABSTRACT:

Background

Lack of a model that mirrors Helicobacter pylori-induced gastric mucosal inflammation has hampered investigation of early host-bacterial interactions. We used an ex vivo model of human stomach, gastric epithelial organoid monolayers (gastroid monolayers) to investigate interactions of H pylori infection and the apical junctional complex and interleukin-8 (IL-8) expression.

Method

Morphology of human antral mucosal gastroid monolayers was evaluated using histology, immunohistochemical (IHC) staining, and transmission electron microscopy (TEM). Functional and gross changes in the apical junctional complexes were assessed using transepithelial electrical resistance (TEER), cytotoxicity assays, and confocal laser scanning microscopy. IL-8 expression was evaluated by r

SUBMITTER: Uotani T 

PROVIDER: S-EPMC6918952 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature