Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations.

ABSTRACT: Viral vectors are complex drugs that pose a particular challenge for manufacturing. Previous studies have shown that, unlike small-molecule drugs, vector preparations do not yield a collection of identical particles. Instead, a mixture of particles that vary in capsid stoichiometry and impurities is created, which may differ from lot to lot. The consequences of this are unclear, but conflicting reports regarding the biological properties of vectors, including transduction patterns, suggest that this variability may have an effect. However, other variables, including differences in animal strains and techniques, make it difficult to identify a cause. Here, we report lot-to-lot variation in spinal cord gray matter transduction following intrathecal delivery of self-complementary adeno-associ

SUBMITTER: O'Connor DM 

PROVIDER: S-EPMC6919242 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature