Development of a novel murine femur fracture and fixation model.

ABSTRACT:

Background

Animal models have been used for decades to simulate human fractures in the laboratory setting. Fracture models in mice are attractive because they offer a high volume, relatively low-cost method of investigating fracture healing characteristics. We report on the development of a novel murine femur fracture model that is rapid, reproducible and inexpensive.

Methods

As part of a pilot study to investigate the effects of smoking on fracture healing, fifteen 35-43 g twelve-week old female CD-1 mice underwent a novel surgical protocol using direct visualization of femur fracture creation and fixation. Following surgery, mice were sacrificed at 14 days, 28 days and 42 days. After sacrifice, the femora were analyzed using MicroCT and histology to evaluate progression of

SUBMITTER: Johnson JP 

PROVIDER: S-EPMC6919350 | biostudies-literature | 2020 Jan-Feb

REPOSITORIES: biostudies-literature