Simultaneous profiling of gene expression and chromatin accessibility in single cells.

ABSTRACT: Profiling multiple omic layers in a single cell enables the discovery and analysis of biological phenomena that are not apparent from analysis of mono-omic data. While methods for multi-omic profiling have been reported, their adoption has been limited due to high cost and complex workflows. Here, we present a simple method for joint profiling of gene expression and chromatin accessibility in tens to hundreds of single cells. We assess the quality of resulting single cell ATAC- and RNA-seq data across three cell types, examine the link between accessibility and expression at the CD3G and FTH1 loci in human primary T cells and monocytes, and compare the accuracy of clustering solutions for mono-omic and combined data. The new method allows biological laboratories to perform si

SUBMITTER: Reyes M 

PROVIDER: S-EPMC6919553 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature