Direct single-molecule quantification reveals unexpectedly high mechanical stability of vinculin-talin/α-catenin linkages.

ABSTRACT: The vinculin-mediated mechanosensing requires establishment of stable mechanical linkages between vinculin to integrin at focal adhesions and to cadherins at adherens junctions through associations with the respective adaptor proteins talin and α-catenin. However, the mechanical stability of these critical vinculin linkages has yet to be determined. Here, we developed a single-molecule detector assay to provide direct quantification of the mechanical lifetime of vinculin association with the vinculin binding sites in both talin and α-catenin, which reveals a surprisingly high mechanical stability of the vinculin-talin and vinculin-α-catenin interfaces that have a lifetime of >1000 s at forces up to 10 pN and can last for seconds to tens of seconds at 15 to 25 pN. Our results suggest that t

SUBMITTER: Le S 

PROVIDER: S-EPMC6920023 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature