Tissue classification by rapid evaporative ionization mass spectrometry (REIMS): comparison between a diathermic knife and CO₂ laser sampling on classification performance.

ABSTRACT: The increasing need for rapid, in situ, and robust tissue profiling approaches in the context of intraoperative diagnostics has led to the development of a large number of ambient ionization-based surface sampling strategies. This paper compares the performances of a diathermic knife and a CO₂ laser handpiece, both clinically approved, coupled to a rapid evaporative ionization mass spectrometry (REIMS) source for quasi-instantaneous tissue classification. Several fresh meat samples (muscle, liver, bone, bone marrow, cartilage, skin, fat) were obtained from different animals. Overall, the laser produced cleaner cuts and more reproducible and higher spectral quality signals when compared with the diathermic knife (CV laser = 9-12%, CV diathermic = 14-23%). The molecular profiles w

SUBMITTER: Genangeli M 

PROVIDER: S-EPMC6920236 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature