Monocytes undergo multi-step differentiation in mice during oral infection by Toxoplasma gondii.

ABSTRACT: Monocytes play a major role in the defense against pathogens. They are rapidly mobilized to inflamed sites where they exert both proinflammatory and regulatory effector functions. It is still poorly understood how this dynamic and exceptionally plastic system is controlled at the molecular level. Herein, we evaluated the differentiation process that occurs in Ly6C^hi monocytes during oral infection by Toxoplasma gondii. Flow cytometry and single-cell analysis revealed distinct activation status and gene expression profiles in the bone marrow, the spleen and the lamina propria of infected mice. We provide further evidence that acquisition of effector functions, such as the capacity to produce interleukin-27, is accompanied by distinct waves of epigenetic programming, highli

SUBMITTER: Detavernier A 

PROVIDER: S-EPMC6920430 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature