TLR5: A prognostic and monitoring indicator for triple-negative breast cancer.

ABSTRACT: A novel, highly selective biomarker is urgently needed to predict and monitor triple-negative breast cancer (TNBC) because targeting molecules are not currently available. Although associated with various malignant tumors, the role of toll-like receptor 5 (TLR5) in TNBC remains uncertain. We aimed to define the effects of TLR5 in TNBC to determine whether it could serve as a prognostic and monitoring indicator for TNBC. We established TNBC cell line 4T1 with low TLR5 expression (GFP tag; TLR5^- 4T1) and with normal TLR5 expression (GFP tag; TLR5⁺ 4T1) using lentivirus-shRNA-TLR5 knockdown transfection and negative lentivirus transfection, respectively. Detected by western blot and qPCR, we found knockdown of TLR5 resulted in decreased expression of TLR5 and E-cadherin

SUBMITTER: Shi D 

PROVIDER: S-EPMC6920449 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature