Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation.

ABSTRACT: The heterotrophic cultivation of microalgae has a number of notable advantages, which include allowing high culture density levels as well as enabling the production of biomass in consistent and predictable quantities. In this study, the full potential of Chlorella sp. HS2 is explored through optimization of the parameters for its heterotrophic cultivation. First, carbon and nitrogen sources were screened in PhotobioBox. Initial screening using the Plackett-Burman design (PBD) was then adopted and the concentrations of the major nutrients (glucose, sodium nitrate, and dipotassium phosphate) were optimized via response surface methodology (RSM) with a central composite design (CCD). Upon validation of the model via flask-scale cultivation, the optimized BG11 medium was found to result in a

SUBMITTER: Kim HS 

PROVIDER: S-EPMC6920485 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature