Polymorphisms of the kappa casein (CSN3) gene and inference of its variants in water buffalo (Bubalus bubalis).

ABSTRACT: Kappa casein plays a crucial role in the formation of stable casein micelles and has a key influence on milk-clotting properties. However, current understanding of buffalo CSN3 gene polymorphisms is not sufficient. In this study, the polymorphisms in the complete coding sequence (CDS) of the buffalo CSN3 were detected using PCR product direct sequencing. The CDS of CSN3 for river and swamp buffalo was the same in length, which contained an open reading frame of 573 nucleotides encoding a peptide containing 190 amino acid residues. A total of eight single nucleotide polymorphisms (SNPs) was identified in two types of buffalo. Among them, c.86C>T, c.252G>C, c.445G>A, c.467C>T and c.516A>C were non-synonymous, which leads to p.Pro8Leu, p.Lys63Asn, p.Val128Ile, p.Thr135Ile and p.Glu151Asp subs

SUBMITTER: Fan X 

PROVIDER: S-EPMC6920614 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature