Digitalizing heterologous gene expression in Gram-negative bacteria with a portable ON/OFF module.

ABSTRACT: While prokaryotic promoters controlled by signal-responding regulators typically display a range of input/output ratios when exposed to cognate inducers, virtually no naturally occurring cases are known to have an OFF state of zero transcription-as ideally needed for synthetic circuits. To overcome this problem, we have modelled and implemented a simple digitalizer module that completely suppresses the basal level of otherwise strong promoters in such a way that expression in the absence of induction is entirely impeded. The circuit involves the interplay of a translation-inhibitory sRNA with the translational coupling of the gene of interest to a repressor such as LacI. The digitalizer module was validated with the strong inducible promoters Pm (induced by XylS in the presence of benzoate

SUBMITTER: Calles B 

PROVIDER: S-EPMC6920698 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature