Phosphorylated Rasal2 facilitates breast cancer progression.

ABSTRACT:

Background

Rasal2 has diametric effects on progression of oestrogen receptor-positive (ER+) and -negative (ER-) breast cancers. The relevant causes are unknown. It is also unknown whether the effects of Rasal2 are mediated by an exosome-transport process.

Methods

Exosomes were purified from breast cancer cells and identified by transmission electron microscopy and flow cytometry analysis. In vivo and in vitro experiments were conducted to investigate the role of Rasal2 in exosome-mediated breast cancer progression. Western blot analysis was performed to detect Rasal2 and p-Rasal2 (phosphorylated Rasal2) expression in ER+/ER- breast cancer cells and in exosomes, cancer tissues and blood of patients with ER+ or ER- breast cancer.

Findings

Phosphorylation of Rasal2 at Se

SUBMITTER: Wang X 

PROVIDER: S-EPMC6921363 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature