PAMAM-cRGD mediating efficient siRNA delivery to spermatogonial stem cells.

ABSTRACT:

Background

Spermatogonial stem cells (SSCs) are the cornerstone of sperm production and thus perpetual male fertility. In clinics, transplantation of patient's own SSCs into testes is a promising technique to restore fertility when male germ cells have been depleted by gonadotoxic therapies. Auto-transplantation of genetically modified SSCs even has the potential to treat male infertility caused by genetic mutations. However, SSCs are refractory to transfection approaches. Poly(amidoamine) (PAMAM) dendrimers have the unique three-dimensional architecture, surface charge, and high density of surface groups that are suitable for ligand attachment, thereby facilitating target delivery. The goal of this study was to elucidate whether PAMAM dendrimers can efficiently deliver short inter

SUBMITTER: Li T 

PROVIDER: S-EPMC6921429 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature