Quantitative proteome analysis of Merkel cell carcinoma cell lines using SILAC.

ABSTRACT:

Background

Merkel cell carcinoma (MCC) is an aggressive neuroendocrine tumour of the skin with growing incidence. To better understand the biology of this malignant disease, immortalized cell lines are used in research for in vitro experiments. However, a comprehensive quantitative proteome analysis of these cell lines has not been performed so far.

Methods

Stable isotope labelling by amino acids in cell culture (SILAC) was applied to six MCC cell lines (BroLi, MKL-1, MKL-2, PeTa, WaGa, and MCC13). Following tryptic digest of labelled proteins, peptides were analysed by mass spectrometry. Proteome patterns of MCC cell lines were compared to the proteome profile of an immortalized keratinocyte cell line (HaCaT).

Results

In total, 142 proteins were upregulated and 43 pr

SUBMITTER: Kotowski U 

PROVIDER: S-EPMC6921584 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature