Noninvasive Imaging and Correlative Histology of Cone Photoreceptor Structure in the Pig Retina.

ABSTRACT:

Purpose

To evaluate different methods of studying cone photoreceptor structure in wild-type (WT) and transgenic pigs carrying the human rhodopsin P23H mutant gene (TgP23H).

Methods

For in vivo imaging, pigs were anesthetized with tiletamine-zolazepam and isoflurane and given lidocaine-bupivacaine retrobulbar injections. Stay sutures and a custom head mount were used to hold and steer the head for adaptive optics scanning light ophthalmoscopy (AOSLO). Six WT and TgP23H littermates were imaged at postnatal day 30 (P30), P90, and P180 with AOSLO and optical coherence tomography (OCT), and two additional sets of littermates were imaged at P3 and P15 with OCT only. AOSLO imaging and correlative differential interference contrast microscopy were performed on a P240 WT pig and on W

SUBMITTER: Huckenpahler AL 

PROVIDER: S-EPMC6922271 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature