Gross transcriptomic analysis of Pseudomonas putida for diagnosing environmental shifts.

ABSTRACT: The biological regime of Pseudomonas putida (and any other bacterium) under given environmental conditions results from the hierarchical expression of sets of genes that become turned on and off in response to one or more physicochemical signals. In some cases, such signals are clearly defined, but in many others, cells are exposed to a whole variety of ill-defined inputs that occur simultaneously. Transcriptomic analyses of bacteria passed from a reference condition to a complex niche can thus expose both the type of signals that they experience during the transition and the functions involved in adaptation to the new scenario. In this article, we describe a complete protocol for generation of transcriptomes aimed at monitoring the physiological shift of P. putida between two divergent se

SUBMITTER: Hueso-Gil A 

PROVIDER: S-EPMC6922523 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature