Standardization of PGC-LC-MS-based glycomics for sample specific glycotyping.

ABSTRACT: Porous graphitized carbon (PGC) based chromatography achieves high-resolution separation of glycan structures released from glycoproteins. This approach is especially valuable when resolving structurally similar isomers and for discovery of novel and/or sample-specific glycan structures. However, the implementation of PGC-based separations in glycomics studies has been limited because system-independent retention values have not been established to normalize technical variation. To address this limitation, this study combined the use of hydrolyzed dextran as an internal standard and Skyline software for post-acquisition normalization to reduce retention time and peak area technical variation in PGC-based glycan analyses. This approach allowed assignment of system-independent retention valu

SUBMITTER: Ashwood C 

PROVIDER: S-EPMC6923133 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature