Identification and Characterization of the Chitin Synthase Genes From the Fish Pathogen Saprolegnia parasitica.

ABSTRACT: Saprolegnia parasitica is a pathogenic oomycete responsible for severe fish infections. Despite its low abundance in the cell wall of S. parasitica, chitin is essential for hyphal growth as the inhibition of its biosynthesis leads to highly reduced growth. Here we identified and characterized chitin synthases (CHS) from S. parasitica as potential targets for anti-oomycete drugs. Bioinformatics analyses allowed the identification of six different putative Chs genes in the genome of the pathogen. The total number of genes was confirmed by Southern blot analysis and their expression levels were determined by quantitative PCR. Four of the six Chs genes were expressed in the mycelium, while the two others exhibited undetectable levels of expression. The myceli

SUBMITTER: Rzeszutek E 

PROVIDER: S-EPMC6923183 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature