Biophysical Control of Bile Duct Epithelial Morphogenesis in Natural and Synthetic Scaffolds.

ABSTRACT: The integration of bile duct epithelial cells (cholangiocytes) in artificial liver culture systems is important in order to generate more physiologically relevant liver models. Understanding the role of the cellular microenvironment on differentiation, physiology, and organogenesis of cholangiocytes into functional biliary tubes is essential for the development of new liver therapies, notably in the field of cholangiophaties. In this study, we investigated the role of natural or synthetic scaffolds on cholangiocytes cyst growth, lumen formation and polarization. We demonstrated that cholangiocyte cyst formation efficiency can be similar between natural and synthetic matrices provided that the mechanical properties of the hydrogels are matched. When using synthetic matrices, we also tried t

SUBMITTER: Funfak A 

PROVIDER: S-EPMC6923240 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature