⁶⁴Cu-ATSM/⁶⁴Cu-Cl₂ and their relationship to hypoxia in glioblastoma: a preclinical study.

ABSTRACT:

Background

Diacetyl-bis(N4-methylthiosemicarbazone), labeled with 64Cu (⁶⁴Cu-ATSM) has been suggested as a promising tracer for imaging hypoxia. However, various controversial studies highlighted potential pitfalls that may disable its use as a selective hypoxic marker. They also highlighted that the results may be tumor location dependent. Here, we first analyzed uptake of Cu-ATSM and its less lipophilic counterpart Cu-Cl₂ in the tumor over time in an orthotopic glioblastoma model. An in vitro study was also conducted to investigate the hypoxia-dependent copper uptake in tumor cells. We then further performed a comprehensive ex vivo study to compare ⁶⁴Cu uptake to hypoxic markers, specific cellular reactions, and also transporter expression.

Met

SUBMITTER: Peres EA 

PROVIDER: S-EPMC6923301 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature