The application of Box-Behnken-Design in the optimization of HPLC separation of fluoroquinolones.

ABSTRACT: Box-Behnken Design is a useful tool for the optimization of the chromatographic analysis. The goal of this study was to select the most significant factors that influenced the following parameters of the chromatographic separation: retention time, relative retention time, symmetry of the peaks, tailing factor, a number of theoretical plates, Foley - Dorsey parameter, resolution factor, peak width at half height. The results underwent the ANOVA test to find the statistically significant variables and interactions between them. The level of significance was for p < 0.05. The polynomial equations described quantitatively the statistically significant parameters and the interactions between them. The statistical analysis indicated both the best conditions for the separation of the compounds an

SUBMITTER: Czyrski A 

PROVIDER: S-EPMC6923357 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature