A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures.

ABSTRACT:

Background

Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be utilized to visualize nucleocapsid transport in Marburg virus (MARV)- or Ebola virus-infected cells, the experiments require biosafety level-4 (BSL-4) laboratories, which are restricted to trained and authorized individuals.

Methods

To overcome this limitation, we developed a live-cell imaging system to visualize MARV nucleocapsid-like structures using fluorescence-conjugated viral proteins, which can be conducted outside BSL-4 laboratories.

Results

Our experiments revealed that nucleocapsid-like structures have similar transport characteristics to those of nucleocapsids observed in MARV-infected cells, bot

SUBMITTER: Takamatsu Y 

PROVIDER: S-EPMC6923871 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature