Genome-wide assessment of DNA methylation in mouse oocytes reveals effects associated with in vitro growth, superovulation, and sexual maturity.

ABSTRACT:

Background

In vitro follicle culture (IFC), as applied in the mouse system, allows the growth and maturation of a large number of immature preantral follicles to become mature and competent oocytes. In the human oncofertility clinic, there is increasing interest in developing this technique as an alternative to ovarian cortical tissue transplantation and to preserve the fertility of prepubertal cancer patients. However, the effect of IFC and hormonal stimulation on DNA methylation in the oocyte is not fully known, and there is legitimate concern over epigenetic abnormalities that could be induced by procedures applied during assisted reproductive technology (ART).

Results

In this study, we present the first genome-wide analysis of DNA methylation in MII oocytes obtained afte

SUBMITTER: Saenz-de-Juano MD 

PROVIDER: S-EPMC6923880 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature