Tissue-specific transposon-associated small RNAs in the gymnosperm tree, Norway spruce.

ABSTRACT:

Background

Small RNAs (sRNAs) are regulatory molecules impacting on gene expression and transposon activity. MicroRNAs (miRNAs) are responsible for tissue-specific and environmentally-induced gene repression. Short interfering RNAs (siRNA) are constitutively involved in transposon silencing across different type of tissues. The male gametophyte in angiosperms has a unique set of sRNAs compared to vegetative tissues, including phased siRNAs from intergenic or genic regions, or epigenetically activated siRNAs. This is contrasted by a lack of knowledge about the sRNA profile of the male gametophyte of gymnosperms.

Results

Here, we isolated mature pollen from male cones of Norway spruce and investigated its sRNA profiles. While 21-nt sRNAs is the major size class of sRNAs in nee

SUBMITTER: Nakamura M 

PROVIDER: S-EPMC6923980 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature