Ontology highlight
ABSTRACT:
SUBMITTER: Chakraborty T
PROVIDER: S-EPMC6924633 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Nature methods 20191031 11
We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleare ...[more]