Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus.

ABSTRACT:

Purpose

To demonstrate the production of inflammatory mediators by cells located in bone marrow spaces inside rodent menisci.

Methods

Mice subjected to transection of the medial collateral and anterior cruciate ligaments and meniscotomy (osteoarthritis model) or to a sham procedure, as well as non-operated (naive) mice and rats, had knee joints excised. Tissues were stained with hematoxylin-eosin and tartrate-resistant acid phosphatase (TRAP). CD68+ cells, inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, and tumor necrosis factor (TNF) expression were detected using immunohistochemistry.

Results

Lamellar ossified areas, bone-entrapped osteocytes and bone marrow spaces were found inside menisci of one week up to 6 months-old naïve mice, regardless of gender

SUBMITTER: Rocha FAC 

PROVIDER: S-EPMC6924665 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature