Multi-omics profiling of mouse gastrulation at single-cell resolution.

ABSTRACT: Formation of the three primary germ layers during gastrulation is an essential step in the establishment of the vertebrate body plan and is associated with major transcriptional changes^1-5. Global epigenetic reprogramming accompanies these changes^6-8, but the role of the epigenome in regulating early cell-fate choice remains unresolved, and the coordination between different molecular layers is unclear. Here we describe a single-cell multi-omics map of chromatin accessibility, DNA methylation and RNA expression during the onset of gastrulation in mouse embryos. The initial exit from pluripotency coincides with the establishment of a global repressive epigenetic landscape, followed by the emergence of lineage-specific epigenetic patterns during gastrulation. Notably, c

SUBMITTER: Argelaguet R 

PROVIDER: S-EPMC6924995 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature