Automated lifespan determination across Caenorhabditis strains and species reveals assay-specific effects of chemical interventions.

ABSTRACT: The goal of the Caenorhabditis Intervention Testing Program is to identify robust and reproducible pro-longevity interventions that are efficacious across genetically diverse cohorts in the Caenorhabditis genus. The project design features multiple experimental replicates collected by three different laboratories. Our initial effort employed fully manual survival assays. With an interest in increasing throughput, we explored automation with flatbed scanner-based Automated Lifespan Machines (ALMs). We used ALMs to measure survivorship of 22 Caenorhabditis strains spanning three species. Additionally, we tested five chemicals that we previously found extended lifespan in manual assays. Overall, we found similar sources of variation among trials for the ALM and our previous manual assays, ver

SUBMITTER: Banse SA 

PROVIDER: S-EPMC6925072 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature