Increasing xylanase activity of Bacillus subtilis by atmospheric pressure plasma jet for biomass hydrolysis.

ABSTRACT: Xylanase producing bacteria, Bacillus subtilis, was bombarded by an atmospheric pressure plasma jet (APPJ) and screened for higher catalytic activity. The bacteria were bombarded with plasma of argon or helium with energy of 120 W for a duration of 1-5 min. A mutant with higher xylanase activity was observed under argon plasma treatment at 1 min on media containing xylan as substrate. Subsequently, the xylanase gene from the mutant was sequenced and named MxynA. Sequence analysis revealed only a single missense mutation on the MxynA gene causing amino acid substitution from threonine to serine at position 162 (T162S) within the xylanase protein of the mutant. Consequently, MxynA was subcloned into expression vector, pETDuet-1 under T7 promoter and expressed in <

SUBMITTER: Polsa N 

PROVIDER: S-EPMC6925083 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature