Cleaved CD31 as a target for in vivo molecular imaging of inflammation.

ABSTRACT: There is a need for new targets to specifically localize inflammatory foci, usable in a wide range of organs. Here, we hypothesized that the cleaved molecular form of CD31 is a suitable target for molecular imaging of inflammation. We evaluated a bioconjugate of D-P8RI, a synthetic peptide that binds all cells with cleaved CD31, in an experimental rat model of sterile acute inflammation. Male Wistar rats were injected with turpentine oil into the gastrocnemius muscle two days before ^99mTc-HYNIC-D-P8RI (or its analogue with L-Proline) SPECT/CT or [¹⁸F]FDG PET/MRI. Biodistribution, stability study, histology, imaging and autoradiography of ^99mTc-HYNIC-D-P8RI were further performed. Biodistribution studies revealed rapid elimination of ^99mTc-HYNIC-D

SUBMITTER: Vigne J 

PROVIDER: S-EPMC6925130 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature