Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli.

ABSTRACT: A balanced and optimized metabolic pathway is the basis for efficient production of a target metabolite. Traditional strategies mostly involve the manipulation of promoters or ribosome-binding sites, which can encompass long sequences and can be complex to operate. In this work, we found that by changing only the three nucleotides of the initiation codons, expression libraries of reporter proteins RFP, GFP, and lacZ with a large dynamic range and evenly distributed expression levels could be established in Escherichia coli (E. coli). Thus, a novel strategy that uses combinatorial modulation of initial codons (CMIC) was developed for metabolic pathway optimization and applied to the three genes crtZ, crtY, and crtI of the zeaxanthin synthesis pathway in E. coli. The initial codons of these

SUBMITTER: Wu Z 

PROVIDER: S-EPMC6925171 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature