Mesenchymal Stromal Cells Induce Podocyte Protection in the Puromycin Injury Model.

ABSTRACT: Podocytes are specialized cells with a limited capacity for cell division that do not regenerate in response to injury and loss. Insults that compromise the integrity of podocytes promote proteinuria and progressive renal disease. The aim of this study was to evaluate the potential renoprotective and regenerative effects of mesenchymal stromal cells (mSC) in a severe form of the podocyte injury model induced by intraperitoneal administration of puromycin, aggravated by unilateral nephrectomy. Bone derived mSC were isolated and characterized according to flow cytometry analyses and to their capacity to differentiate into mesenchymal lineages. Wistar rats were divided into three groups: Control, PAN, and PAN+ mSC, consisting of PAN rats treated with 2 × 10⁵ mSC. PAN rats developed

SUBMITTER: Ornellas FM 

PROVIDER: S-EPMC6925195 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature