Intestinal Neurod1 expression impairs paneth cell differentiation and promotes enteroendocrine lineage specification.

ABSTRACT: Transcription factor Neurod1 is required for enteroendocrine progenitor differentiation and maturation. Several earlier studies indicated that ectopic expression of Neurod1 converted non- neuronal cells into neurons. However, the functional consequence of ectopic Neurod1 expression has not been examined in the GI tract, and it is not known whether Neurod1 can similarly switch cell fates in the intestine. We generated a mouse line that would enable us to conditionally express Neurod1 in intestinal epithelial cells at different stages of differentiation. Forced expression of Neurod1 throughout intestinal epithelium increased the number of EECs as well as the expression of EE specific transcription factors and hormones. Furthermore, we observed a substantial reduction of Paneth cell marker ex

SUBMITTER: Li HJ 

PROVIDER: S-EPMC6925293 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature