Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets.

ABSTRACT: Genome-scale CRISPR-Cas9 viability screens performed in cancer cell lines provide a systematic approach to identify cancer dependencies and new therapeutic targets. As multiple large-scale screens become available, a formal assessment of the reproducibility of these experiments becomes necessary. We analyze data from recently published pan-cancer CRISPR-Cas9 screens performed at the Broad and Sanger Institutes. Despite significant differences in experimental protocols and reagents, we find that the screen results are highly concordant across multiple metrics with both common and specific dependencies jointly identified across the two studies. Furthermore, robust biomarkers of gene dependency found in one data set are recovered in the other. Through further analysis and replication experime

SUBMITTER: Dempster JM 

PROVIDER: S-EPMC6925302 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature