Lysyl-tRNA Synthetase from Pseudomonas aeruginosa: Characterization and Identification of Inhibitory Compounds.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial infections and has highly developed systems for acquiring resistance against numerous antibiotics. The gene (lysS) encoding P. aeruginosa lysyl-tRNA synthetase (LysRS) was cloned and overexpressed, and the resulting protein was purified to 98% homogeneity. LysRS was kinetically evaluated, and the K_m values for the interaction with lysine, adenosine triphosphate (ATP), and tRNA^Lys were determined to be 45.5, 627, and 3.3 µM, respectively. The k_cat^obs values were calculated to be 13, 22.8, and 0.35 s^-1, resulting in k_cat^obs/K_M values of 0.29, 0.036, and 0.11 s^-1µM^-1</

SUBMITTER: Balboa S 

PROVIDER: S-EPMC6925310 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature