Regulation underlying hierarchical and simultaneous utilization of carbon substrates by flux sensors in Escherichia coli.

ABSTRACT: Many microorganisms exhibit nutrient preferences, exemplified by the 'hierarchical' consumption of certain carbon substrates. Here, we systematically investigate under which physiological conditions hierarchical substrate utilization occurs and its mechanisms of implementation. We show utilization hierarchy of Escherichia coli to be ordered by the carbon-uptake flux rather than the identity of the substrates. A detailed study of glycerol uptake finds that it is fully suppressed if the uptake flux of another glycolytic substrate exceeds a threshold, which is set to the influx obtained when grown on glycerol alone. Below this threshold, limited glycerol uptake is 'supplemented' such that the total carbon uptake is maintained at the threshold. This behaviour results from total-flux feedback m

SUBMITTER: Okano H 

PROVIDER: S-EPMC6925339 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature