Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

ABSTRACT:

Background

Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb.

Methods

From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers.

SUBMITTER: Khodaverdi M 

PROVIDER: S-EPMC6925478 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature