Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition.

ABSTRACT: DNA, when folded into nanostructures with a specific shape, is capable of spacing and arranging binding sites into a complex geometric pattern with nanometre precision. Here we demonstrate a designer DNA nanostructure that can act as a template to display multiple binding motifs with precise spatial pattern-recognition properties, and that this approach can confer exceptional sensing and potent viral inhibitory capabilities. A star-shaped DNA architecture, carrying five molecular beacon-like motifs, was constructed to display ten dengue envelope protein domain III (ED3)-targeting aptamers into a two-dimensional pattern precisely matching the spatial arrangement of ED3 clusters on the dengue (DENV) viral surface. The resulting multivalent interactions provide high DENV-binding avidity. We s

SUBMITTER: Kwon PS 

PROVIDER: S-EPMC6925649 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature