Polarized PtdIns(4,5)P₂ distribution mediated by a voltage-sensing phosphatase (VSP) regulates sperm motility.

ABSTRACT: The voltage-sensing phosphatase (VSP) is a unique protein that shows voltage-dependent phosphoinositide phosphatase activity. Here we report that VSP is activated in mice sperm flagellum and generates a unique subcellular distribution pattern of PtdIns(4,5)P₂ Sperm from VSP^-/- mice show more Ca²⁺ influx upon capacitation than VSP^+/- mice and abnormal circular motion. VSP-deficient sperm showed enhanced activity of Slo3, a PtdIns(4,5)P₂-sensitive K⁺ channel, which selectively localizes to the principal piece of the flagellum and indirectly enhances Ca²⁺ influx. Most interestingly, freeze-fracture electron microscopy analysis indicates that normal sperm have much less PtdIns(4,5)P₂ in the principal piece

SUBMITTER: Kawai T 

PROVIDER: S-EPMC6925991 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature