Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineered Escherichia coli.

ABSTRACT: Metabolic engineering seeks to reprogram microbial cells to efficiently and sustainably produce value-added compounds. Since chemical production can be at odds with the cell's natural objectives, strategies have been developed to balance conflicting goals. For example, dynamic regulation modulates gene expression to favor biomass and metabolite accumulation at low cell densities before diverting key metabolic fluxes toward product formation. To trigger changes in gene expression in a pathway-independent manner without the need for exogenous inducers, researchers have coupled gene expression to quorum-sensing (QS) circuits, which regulate transcription based on cell density. While effective, studies thus far have been limited to one control point. More challenging pathways may require layer

SUBMITTER: Dinh CV 

PROVIDER: S-EPMC6926038 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature