Engineering geometrical 3-dimensional untethered in vitro neural tissue mimic.

ABSTRACT: Formation of tissue models in 3 dimensions is more effective in recapitulating structure and function compared to their 2-dimensional (2D) counterparts. Formation of 3D engineered tissue to control shape and size can have important implications in biomedical research and in engineering applications such as biological soft robotics. While neural spheroids routinely are created during differentiation processes, further geometric control of in vitro neural models has not been demonstrated. Here, we present an approach to form functional in vitro neural tissue mimic (NTM) of different shapes using stem cells, a fibrin matrix, and 3D printed molds. We used murine-derived embryonic stem cells for optimizing cell-seeding protocols, characterization of the resulting internal structure of the const

SUBMITTER: Pagan-Diaz GJ 

PROVIDER: S-EPMC6926042 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature