Structures of Respiratory Supercomplex I+III₂ Reveal Functional and Conformational Crosstalk.

ABSTRACT: The mitochondrial electron transport chain complexes are organized into supercomplexes (SCs) of defined stoichiometry, which have been proposed to regulate electron flux via substrate channeling. We demonstrate that CoQ trapping in the isolated SC I+III₂ limits complex (C)I turnover, arguing against channeling. The SC structure, resolved at up to 3.8 Å in four distinct states, suggests that CoQ oxidation may be rate limiting because of unequal access of CoQ to the active sites of CIII₂. CI shows a transition between "closed" and "open" conformations, accompanied by the striking rotation of a key transmembrane helix. Furthermore, the state of CI affects the conformational flexibility within CIII₂, demonstrating crosstalk between the enzymes. CoQ was identifi

SUBMITTER: Letts JA 

PROVIDER: S-EPMC6926478 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature