Engineered Pseudomonas putida KT2440 co-utilizes galactose and glucose.

ABSTRACT:

Background

Efficient conversion of plant biomass to commodity chemicals is an important challenge that needs to be solved to enable a sustainable bioeconomy. Deconstruction of biomass to sugars and lignin yields a wide variety of low molecular weight carbon substrates that need to be funneled to product. Pseudomonas putida KT2440 has emerged as a potential platform for bioconversion of lignin and the other components of plant biomass. However, P. putida is unable to natively utilize several of the common sugars in hydrolysate streams, including galactose.

Results

In this work, we integrated a De Ley-Doudoroff catabolic pathway for galactose catabolism into the chromosome of P. putida KT2440, using genes from several different organisms. We found that the

SUBMITTER: Peabody GL 

PROVIDER: S-EPMC6927180 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature