M⁶A mRNA methylation regulates CTNNB1 to promote the proliferation of hepatoblastoma.

ABSTRACT:

Background

N⁶-Methyladenosine (m⁶A) modification has been implicated in many biological processes. It is important for the regulation of messenger RNA (mRNA) stability, splicing, and translation. However, its role in cancer has not been studied in detail. Here we investigated the biological role and underlying mechanism of m⁶A modification in hepatoblastoma (HB).

Methods

We used Reverse transcription quantitative real-time PCR (RT-qPCR) and Western blotting to determine the expression of m⁶A related factors. And we clarified the effects of these factors on HB cells using cell proliferation assay, colony formation, apoptotic assay. Then we investigated of methyltransferase-like 13 (METTL3) and its correlation with clinicopathologica

SUBMITTER: Liu L 

PROVIDER: S-EPMC6927193 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature