Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles.

ABSTRACT:

Objective

Neutrophil infiltration and release of the abundant human neutrophil peptides (HNP) are a common clinical feature in critically ill patients. We tested a hypothesis that different cell types respond to HNP differently in lung microenvironment that may influence the host responses.

Methods

Plasma concentrations of HNP were measured in healthy volunteers and patients with sepsis. Cells including the bacteria P. aeruginosa, human lung epithelial cells and mesenchymal stromal cells (MSCs) were exposed to various concentrations of HNP. Bacterial killing, epithelial cell inflammation, MSC adhesion and behaviours were examined after HNP stimulation.

Results

Incubation of P. aeruginosa or stimulation of human lung epithelial cells with HNP resulted in

SUBMITTER: Dai Q 

PROVIDER: S-EPMC6927223 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature