Real time dynamics of Gating-Related conformational changes in CorA.

ABSTRACT: CorA, a divalent-selective channel in the metal ion transport superfamily, is the major Mg²⁺-influx pathway in prokaryotes. CorA structures in closed (Mg²⁺-bound), and open (Mg²⁺-free) states, together with functional data showed that Mg²⁺-influx inhibits further Mg²⁺-uptake completing a regulatory feedback loop. While the closed state structure is a symmetric pentamer, the open state displayed unexpected asymmetric architectures. Using high-speed atomic force microscopy (HS-AFM), we explored the Mg²⁺-dependent gating transition of single CorA channels: HS-AFM movies during Mg²⁺-depletion experiments revealed the channel's transition from a stable Mg²⁺-bound state over a highly mobile and dynamic state

SUBMITTER: Rangl M 

PROVIDER: S-EPMC6927688 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature